High resolution analysis of organic 3D nano-crystals.

High resolution analysis of organic 3D nano-crystals.
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Jan-Pieter Abrahams

In collaboration with ASI we developed a fast quantum area detector. This novel detector, mounted on a transmission electron microscope, allows acquiring full 3D structural information at atomic resolution, using just a few crystals with a diameter of 0.0002 mm or less. This breakthrough allows visualizing molecular structures important for life science and pharmaceutical research, that hitherto could not be studied because of their failure to grow large crystals.

3D datasets

During the last months we collected a vast amount of electron diffraction patterns with our quantum electron detector at the University of Delft (The Netherlands), the University of Barcelona (Spain), the Ernst Ruska Centre for Microscopy and Spectroscopy (ERC) in Juelich (Germany) and the Nederlands Center for Electron Nanoscopy (NeCEN) in Leiden (The Netherlands). A selection of 3D reciprocal space reconstructions of three organic crystals; Salicylic Acid, Nicotinic Acid and Antenna Complex can be viewed below. Also an electron diffraction data set (70°) of Carbamazepine is shown.

Salicylic Acid
High resolution analysis of organic 3D nano-crystals.

An image of a 100nm thin Salicylic Acid crystal and its structural formula C7H6O3.

The video below shows a 3D reciprocal space reconstruction of Salicyclic Acid (500x500x100nm3).

Nicotinic Acid
High resolution analysis of organic 3D nano-crystals.

An image of a 200nm thin Nicotinic Acid crystal and its structural formula C6H5NO2.

The video below shows a 3D reciprocal space reconstruction of Nicotinic Acid 3D (700x900x200nm3).

Antenna Complex
High resolution analysis of organic 3D nano-crystals.

An image of an Antenna Complex crystal and its structural formula C90H81N3O10S2.

The video below shows a 3D reciprocal space reconstruction of the Antenna Complex (200x600x150nm3).

Carbamazepine
High resolution analysis of organic 3D nano-crystals.

An image of a 200nm thin Carbamazepine crystal and its structural formula C15N2OH12.

The video below shows an electron diffraction data set of Carbemazopine (70°). The data were acquired with a quantum area detector mounted on a CM30 electron microscope at 200keV at room temperature.

Structure determination
High resolution analysis of organic 3D nano-crystals.

The structure solution of Carbamazepine. Five data sets of rotation electron diffraction patterns were used to determine the unit cell. The structure was solved by direct methods using SHELXT and refined with SHELXL.

20/03/2015