Bio-imaging technology for better diagnostic tools
WP1.1 Towards near atomic resolution for single-particle cryo-electron microscopy
X-ray crystallography is the commonly used method for unravelling the 3D structures of proteins. Only about 8% of proteins crystallise and only 3% produce crystals big enough to solve with this technology. We want to optimise cryo-transmission electron microscopy to (i) solve the atomic structures of proteins that only produce nano-crystals and (ii) determine intermediate resolution structures (0.4 to 0.3 nm resolution) of protein complexes that fail to crystallise. We focus on electron detection, microscope data generation and data analysis.
Figure: Inside of the NeCEN Titan Krios cryo-transmission electron microscope