Bio-imaging technology for better diagnostic tools
WP1.2 FIB-SEM and correlative electron microscopy
In order to understand diseases and diagnoses, it is important to find specific cells in an overview image and then zoom in to high magnifications for detailed information. This can be achieved by correlative microscopy. We will work on two types of correlative microscopy: 1. Focused-Ion-Beam-Scanning-Electron Microscopy (FIB-SEM) and 2. the combination of Transmission Electron Microscopy (TEM) with fluorescence microscopy. We focus on further developing and applying these types of correlative microscopy in the vascular field.
Figure: Typical microscopy images of combined transmission electron microscopy (TEM) and fluorescence microscopy (FM).