Bio-imaging technology for better diagnostic tools
WP1.3 New tools for integrated, high-throughput, high-resolution imaging
In this work package we will identify genes and study processes associated with inflammation cancer progression and cardiovascular diseases. This information is anticipated to lead to more accurate prognosis, diagnosis and/or staging of the diseases. We will use and develop various microscopic techniques, including high-resolution microscopy (STED micoscopy), high throughput high content microscopy (automated confocal microscopy) and intravital live cell imaging in the intact organisms (multiphoton microscopy). This will be combined with high throughput screening devices for studying diseases in zebrafish embryos and RNAi screens for identifying disease-related genes. We will also investigate responses to disease-related chemical gradients. Integrating the different technologies is expected to lead to valuable new scientific insights with great potential for novel drug target identification.
Figure: The spread of cancer cells (red) in a fluorescent zebrafish embryo (green). Top: whole embryo, bottom: close-up showing metastasis in detail. Image from VPS Ghotra, S He, H de Bont, W van der Ent, HP Spaink, B van de Water, BE Snaar-Jagalska, EHJ Danen. Automated whole animal bio-imaging assay for human cancer dissemination. PloS One, in press 2012.